From a maize field plot planted in May 2017 at the ILVO fields in Wetteren (Belgium), ears of the genotype CML91, H99, W153R, and OH43 were harvested at the end of June. Late cobs, leaves, stems (internodium that bears the maize cob), and tassels from these four genotypes were harvested at the end of August. The 20 samples (four genotypes and five organ types, no biological replicates were included) were separately homogenized using a GRINDOMIX GM 200 (Retch GmbH, Germany). Approximately 200 mg fresh weight was extracted with 1 mL methanol. Following evaporation of the methanol supernatant, an extraction with 0.8 mL of Milli-Q water/cyclohexane (1/1, v/v) was performed as previously described [39]. Finally, 0.2 mL of the aqueous phase was stored at −80 °C.

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