2.3.1. Animals in Experiments 1 and 2
This protocol is extracted from research article:
Dataset of reaching behavior for reward in social situations in mice
Data Brief, Jan 30, 2021; DOI: 10.1016/j.dib.2021.106773

For Experiment 1, 32 male C57BL/N mice (CREA Japan Inc.), with a mean weight of 21.25 g (SD: 1.12 g) and an age of 6 weeks at the time of purchase, were separated into groups of four mice and housed in a temperature-controlled (approximately 23 °C) animal room under a 12 h light/dark cycle (light from 8:00 AM to 20:00 PM) (Table 1). Before the experiments, the mice were provided with food (CE-2, CREA Japan, Inc.) and tap water ad libitum as preliminary breeding for one week. During the behavioral experiments, approximately 1 g of food per day was provided to each mouse after the day's experiment. Tap water was continuously available in their home cages.

For Experiment 2, 50 male C57BL/N mice (CREA Japan Inc.), with a mean weight of 19.25 g (SD: 1.39 g) and an age of 5–7 weeks at the time of purchase, were separated into groups of two or four mice (Table 8 and Table 9). We randomly assigned ten animals to the Learning group, ten to the Unlearning group, and ten to the Empty group. They were all trained in reaching. In addition, twenty animals were prepared as demonstrators of learned reaching or unlearned reaching in the reaching room for the test session. The demonstrators for the unlearned reaching group only exhibited the unlearned reaching behavior in the test session. Before the experiments, the treatment during preliminary breeding was the same as that in Experiment 1, except that the duration was 1–14 days. During the experiment, the feeding and water restriction schedule was the same as that in Experiment 1.

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