Genomic DNA (gDNA) was extracted from ovarian tissues using a QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. Approximately 1 μg of high-quality gDNA was bisulfite-converted using the EZ DNA methylation kit (Zymo Research, Cambridge Bioscience, Cambridge, United Kingdom) according to the manufacturer’s instructions. Genome-wide DNA methylation profiles were generated with an Infinium Methylation EPIC BeadChip Array (Illumina, San Diego, CA, United States). Bisulfite-converted DNA was used for the whole-genome amplification reaction, enzymatic fragmentation, precipitation, and resuspension in hybridization buffer. Subsequent steps were carried out according to the standard Infinium HD Assay Methylation Protocol Guide. The BeadChip images were captured using the Illumina iScan system (Illumina, San Diego, CA, United States).

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