Two days after IVA and 1 month after transplantation, some ovaries were fixed with formalin, paraffin-embedded, and then cut into continuous 8-μm-thick sections. One fragment was collected for calculation of the number of follicles and the experiment was repeated three times. Sections were stained with hematoxylin and eosin (HE) reagent for further observation. Then we selected one slide of every five and each sample was chosen 15 pieces in the lump to calculate total follicles and the percentage of different phases of follicles to total follicles, thus avoiding counting the same slide twice or missing a follicle.

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