Two days after IVA and 1 month after transplantation, some ovaries were fixed with formalin, paraffin-embedded, and then cut into continuous 8-μm-thick sections. One fragment was collected for calculation of the number of follicles and the experiment was repeated three times. Sections were stained with hematoxylin and eosin (HE) reagent for further observation. Then we selected one slide of every five and each sample was chosen 15 pieces in the lump to calculate total follicles and the percentage of different phases of follicles to total follicles, thus avoiding counting the same slide twice or missing a follicle.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.