Pairs of ovarian tissue were cultured for 3 h, and proteins were extracted using RIPA buffer containing a protease inhibitor cocktail (APExBIO, United States) and phosphatase inhibitors (Roche, Switzerland). Protein concentrations were determined by BCA protein assay (Beyotime Institute of Biotechnology, China). Equal amounts of protein lysates were separated on 12% PAGE Bis-Tris gels (Epizyme) and transferred onto nitrocellulose membranes. Proteins were visualized, and images were obtained using an Odyssey infrared imager.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.