Phylogenetic analyses were required to reveal historical relationships among taxa and to test the validity of the prevailing species hypotheses. Maximum likelihood (ML) trees for ddRADseq datasets were inferred with the IQ-Tree program94 with 5000 ultrafast bootstrap support for branches95. Haplotype networks for COI were computed with a modified script in RStudio (originally from using packages ape/5.396 and pegas/0.1197 and PopArt/1.7 using the TCS method98,99. Maximum likelihood trees for COI datasets were constructed using MEGA7100 with bootstrap support estimated from 500 replicates and the GTR + G substitution model.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.