The plant materials of Z. piasezkii, Z. armatum, Z. motuoense, and Z. oxyphyllum were collected from Nyingchi (Tibet, China); Z. multijugum and Z. calcicola were obtained from Kunming (Yunnan, China). Fresh, healthy leaves were directly dried with silica gel after collection. Total genomic DNA was isolated using a modified CTAB method (Li et al., 2013). The DNA integrity and concentration were measured using agarose gel electrophoresis and a NanoDrop 2000 Spectrophotometer (Thermo Scientific, Carlsbad, CA, United States). Purified DNA was randomly sheared into fragments using physical methods. Paired-end reads (150 bp) were generated on an Illumina HiSeq X 10 System (San Diego, CA, United States). Total genomic DNAs were also sent to BGI (Shenzhen, China) for library (400 bp) preparation for genome skimming sequencing. Paired-end (150 bp) sequencing was conducted on the Illumina HiSeq X-10 platform, generating ∼2 Gb data per sample. Low-quality sequences were filtered by NGS QC Toolkit v2.3.333 (Patel and Jain, 2012) with Q30 (base Phred quality score of ≥ 30) was used to obtain high-quality reads.

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