Heart samples were sectioned (5 μm thick) and stained using picrosirius red for quantification of interstitial collagen. Stained cross sections were captured using light microscopy (Eclipse 50i, Nikon, Tokyo, Japan) at 40× magnification. To estimate the fraction area (percentage) of collagen using picrosirius red–stained sections, 15 to 20 images of the septum were randomly captured for posterior analysis using ImageJ software (National Institutes of Health, Bethesda, Maryland). LV infarct size was measured using picrosirius red–stained sections and calculated by dividing the length of the infarcted area by the total circumference of the left ventricle (expressed as a percentage). Only cells with well-defined cell membranes and visible cell nuclei at midwall depth were selected to measure cardiomyocyte diameter on sections stained with Masson’s trichrome.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.