Five-day-old Arabidopsis seedlings of all lines grown in 1/2 MS medium were collected for RNA extraction. Quantitative reverse transcription PCR (RT-qPCR) was performed using primers targeting genes related to trichome differentiation, including GLABRA 1 (AT3G27920, AtGL1), WD40 REPEAT-LIKE SUPERFAMILY PROTEIN (AT5G24520, AtTTG1), SUPER SENSITIVE TO ABA AND DROUGHT 2 (AT2G31660, AtSAD2), SUPER SENSITIVE TO ABA AND DROUGHT 1 (AT5G48870, AtSAD1), GLABRA3 (AT5G41315, AtGL3), ENHANCER OF GLABRA3 (AT1G63650, AtEGL3), CAPRICE (AT2G46410, AtCPC), and TRYPTICHON (AT5G53200, AtTRY), which are listed as genename-S and genename-A (for example, AtTTG1-S, and AtTTG1-A) in Supplementary Table 1.

RNA extraction was performed on seedlings treated with 0 and 100 mM for 1 week. Three stress-related marker genes were selected for RT-qPCR: SALT OVERLY SENSITIVE 1 (AT2G01980, AtSOS1), Δ1-PYRROLINE-5-CARBOXYLATE SYNTHETASE 1 (AT2G39800, AtP5CS1), and GST CLASS TAU 5 (AT2G2945, AtGSTU5) (Supplementary Table 1). Three biological replicate experiments were performed. Relative expression levels were calculated using the formula 2–△ C(T). AtACTIN2 (primers ACTIN2 sense and ACTIN2 anti) was used as an internal control.

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