BCCAO operation in vivo is a classic and an acknowledged model for the study of molecular mechanisms of CCH and its interventions (Zhang et al., 2017; Zou et al., 2018; Li et al., 2019b; Mao et al., 2019). Rats were anesthetized with 3% sodium pentobarbital (50 mg/kg, intraperitoneal (i.p.) injection; Li et al., 2019b,c). CCH was induced using minimally invasive BCCAO operation (Farkas et al., 2007; Zhang et al., 2017; Zou et al., 2018; Mao et al., 2019). The detailed surgical procedures have been described previously (Li et al., 2019b,c; Su et al., 2019). Briefly, a ventral incision was made in the midline of the neck with a scalpel, bilateral common carotid arteries were exposed and gently separated from the vagus nerve, then permanently occluded with 4–0 silk suture in the BCCAO group. The sham rats were subjected to the same procedure without occlusion of both vessels. The body temperature of the rats was maintained using a heating lamp during the surgical procedure. After the operation, the rats were housed in an animal resource facility with ad libitum access to food and water.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.