As a common visitor of S. arvensis, we used Bombus terrestris L. (Apidae) from self-reared colonies at the Institute of Evolutionary Ecology and Conservation Genomics at Ulm University (Rottler et al., 2013; Rottler-Hoermann et al., 2016) for our behavioral assays. The founding queens were descendants of commercial colonies (Koppert Biological Systems, Netherlands). The colonies were kept in wooden boxes (39 cm × 16.5 cm × 16 cm) in constant darkness at a temperature of 27°C and a relative humidity of 60%. The bumblebees were provided ad libitum with a 55% sugar solution of API-Invert® (Südzucker AG, Mannheim, Germany) and fresh pollen (Koppert Biological Systems, Netherlands).

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