The LPO end product malondialdehyde (MDA) concentration was measured in serum and brain supernatant using the thiobarbituric reacting substance (TBARS) assay [43]. Reduced glutathione (GSH) concentration in the serum and brain supernatant was determined using the Ellman’s reagent [44]. Assay for catalase enzyme activity in serum and brain supernatants of was determined using the colorimetric assay based on the yellow complex formation with H2O2 and molybdate [45].

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