Samples collection and fungal strains isolation
This protocol is extracted from research article:
One new species and one new record of Zasmidium in China
Biodivers Data J, Jan 7, 2021; DOI: 10.3897/BDJ.9.e59001

The samples were collected in Xishuangbanna City, Yunnan Province, China. In order to obtain pure cultures, diseased leaf pieces of Smilax china (Liliales, Smilacaceae) and Cremastra appendiculata (Asparagales, Orchidaceae) were surface-disinfected following the method of Zhang et al. (2020). The strains were isolated using the single-spore method (Chomnunti et al. 2014). Colonies growing from single spores were transferred to potato-dextrose agar (PDA) and incubated at room temperature (28ºC). The holotype was deposited in the Herbarium of Department of Plant Pathology, Agricultural College, Guizhou University (HGUP). The ex-type cultures were deposited in the Culture Collection at the Department of Plant Pathology, Agriculture College, Guizhou University, P.R. China (GUCC) and the Mae Fah Luang University Culture Collection (MFLUCC) in Thailand.

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