Animals used were 28 male Wistar rats of about 70–90 g after one week acclimatization in the experimental animal house, Department of Pharmacology and Therapeutics, University of Ibadan. The animals were kept in good hygienic conditions in polypropylene plastic cages with wood shavings as bedding. All animals were allowed free access to water and fed with standard commercial rat chow pellets (Vital feeds Ltd, Ibadan, Nigeria) for animals on basal diet or formulated high-fat diet for the other experimental groups. All experiments were carried out with strict compliance to The “Principle of Laboratory Animal Care” (NIH Publication No. 85–23) and approved institutional protocols on animal handling.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.