Animals used were 28 male Wistar rats of about 70–90 g after one week acclimatization in the experimental animal house, Department of Pharmacology and Therapeutics, University of Ibadan. The animals were kept in good hygienic conditions in polypropylene plastic cages with wood shavings as bedding. All animals were allowed free access to water and fed with standard commercial rat chow pellets (Vital feeds Ltd, Ibadan, Nigeria) for animals on basal diet or formulated high-fat diet for the other experimental groups. All experiments were carried out with strict compliance to The “Principle of Laboratory Animal Care” (NIH Publication No. 85–23) and approved institutional protocols on animal handling.

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