5′, 5′-Dithiobis 2-nitrobenzoate (DTNB), Trichloroacetic acid (TCA), Thiobarbituric acid (TBA), Griess reagent ((sulfanilamide and N-(1-naphthyl) ethylenediamine dihydrochloride), were all products of Sigma-Aldrich St. Louis, USA. Interleukin −6 Biolegend ELISA kits (San Diego, USA). All other solvents and reagents were analytical grade reagents.

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