After transfection, the treated or control cells were lysed with lysis buffer containing protease inhibitors. The protein samples were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and then transferred to polyvinylidene fluoride membranes. After blocking with 5% defatted milk, the membranes were incubated with primary antibodies against MUC8 (Santa Cruz Biotechnology, Beijing, China), MUC5AC (ab198294, Abcam, USA), p65 (ab16502, Abcam), Lamin B (ab194109, Abcam), β-tubulin (ab6046, Abcam), IκBα (ab32518, Abcam), p-IκBα (ab133462, Abcam), and β-actin (ab8227, Abcam) at a dilution of 1 : 1000 at 4°C overnight. Following washing with Tris-Buffered Saline Tween-20 (TBST), the membranes were exposed to HRP-conjugated goat anti-rabbit (1 : 5000, Abcam) secondary antibodies. At last, protein bands were detected with an ECL detection system and quantified by ImageJ. Each experiment was repeated three times.

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