2.5. Factors Affecting the Ability of L. acidophilus NCFM to Bind B(a)P

For either of the simulated inorganic or organic system, L. acidophilus NCFM was grown in both systems for the observation of its B(a)P binding ability under different incubation times at 37°C. After incubated in two simulated systems for 10 min, 60 min, 240 min, 480 min, 1080 min, and 1440 min, respectively, the percentage of strain NCFM to bind B(a)P was detected.

For either of the simulated inorganic or organic system, L. acidophilus NCFM was cultivated in the two simulated systems for the observation of its B(a)P binding ability under different incubation temperatures. The ability of L. acidophilus NCFM to bind B(a)P was evaluated after this strain was incubated at 4°C, 15°C, 23°C, and 37°C for 8 hr, respectively.

For either of the simulated inorganic or organic system, L. acidophilus NCFM was cultured in the two simulated systems for the observation of its B(a)P binding ability under different pH values at 37°C. The ability of L. acidophilus NCFM to bind B(a)P was evaluated after this strain was incubated at pH 3, 4, 5, 6, 7, 8, and 9 for 8 hr, respectively. The sample was adjusted to a specific pH value using a degassed phosphate buffer solution.

For either of the simulated inorganic or organic system, L. acidophilus NCFM was grown in the two simulated systems for the observation of its B(a)P binding ability under cell concentrations. L. acidophilus NCFM was inoculated into the two simulated systems at cell concentrations of 1 × 108 cfu/mL, 1 × 109 cfu/mL, and 1 × 1010 cfu/mL, respectively. Estimation of cell concentrations during cultures was done by turbidimetry [20]. After incubated at 37°C for 8 hr, the ability of this strain to bind B(a)P was evaluated in terms of its cell concentrations.

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