2.5.4. Effect of Cell Concentration

For either of the simulated inorganic or organic system, L. acidophilus NCFM was grown in the two simulated systems for the observation of its B(a)P binding ability under cell concentrations. L. acidophilus NCFM was inoculated into the two simulated systems at cell concentrations of 1 × 108 cfu/mL, 1 × 109 cfu/mL, and 1 × 1010 cfu/mL, respectively. Estimation of cell concentrations during cultures was done by turbidimetry [20]. After incubated at 37°C for 8 hr, the ability of this strain to bind B(a)P was evaluated in terms of its cell concentrations.

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