For either of the simulated inorganic or organic system, L. acidophilus NCFM was cultured in the two simulated systems for the observation of its B(a)P binding ability under different pH values at 37°C. The ability of L. acidophilus NCFM to bind B(a)P was evaluated after this strain was incubated at pH 3, 4, 5, 6, 7, 8, and 9 for 8 hr, respectively. The sample was adjusted to a specific pH value using a degassed phosphate buffer solution.

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