Acute peritonitis mice were generated using C57BL/6 mice (10 mice/group) as previously reported (Yi et al. 2016). Sb-EE (200 mg/kg) suspended in 0.5% sodium carboxymethylcellulose (CMC) was orally administered once per day for five days. Acute peritonitis was induced with an intraperitoneal injection of 1.0 mL (10 mg/kg) LPS on day 4 after Sb-EE administration. Peritoneal fluid was harvested by intraperitoneal lavage using sterile PBS on day 5. In peritoneal macrophages obtained from peritonitis mice, NO production and phosphorylation pattern of target proteins were analysed using an NO assay and immunoblotting, respectively.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.