The artificial inorganic system (100 μL) plus 900 μL sterilized water, containing 1.0 μg/mL B(a)P, was added to 1.0 mL bacterial suspension. After incubation at 37°C for 4 hr, the supernatant was collected by centrifugation (3000 r/min, 5 min). Chloroform (500 μL) was added to the supernatant to produce the organic phase for the detection of B(a)P. The control was designed as 1.0 μg/mL aqueous solution of B(a)P without bacterial cell addition. For each sample, B(a)P was detected by HPLC with the following conditions: UV detection of 290 nm wavelength, mobile phase of pure methanol, selection of room temperature as column temperature, flow rate of 1.0 mL/min, and injection volume of 20.0 μL [18].

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