2.3. Preparation of Two B(a)P Simulation Systems

The PM2.5-based inorganic compositions simplify the artificial inorganic system. Artificial inorganic system (1.0 mL) consisting of 100 μL of B(a)P working solution (10 μg/mL), 300 μL of sodium sulfate solution (100 μg/mL), 300 μL of ammonium sulfate solution (100 μg/mL), and 100 μL of sterile water was designed according to Dhananjay et al. with some modifications [5].

Regarding the organic compositions of PM2.5 which belong to the family PHAs, PHA Mix (EPA 610), purchased from Accredited Chemical Testing Lab with a purity ≥98.5%, was used to simulate the organic system of B(a)P. The PHA Mix consists of 16 various concentrations of organic compounds, including benzo[k]fluoranthene of 99.37 ± 1.4 mg/L, chrysene of 99.59 ± 1.41 mg/L, phenanthrene of 98.9 ± 1.31 mg/L, benzo[b]fluoranthene of 202.3 ± 2.69 mg/L, benzo[ghi]perylene of 200.6 ± 2.67 mg/L, fluoranthene of 198.3 ± 2.64 mg/L, fluorine of 200.4 ± 2.66 mg/L, naphthalene of 1000 ± 3.74 mg/L, acenaphthene of 991 ± 6.1 mg/L, acenaphthylene of 2018 ± 12.31 mg/L, anthracene of 101.2 ± 1.34 mg/L, benzo[a]anthracene of 99.98 ± 1.41 mg/L, benzo[a]pyrene of 100.8 ± 1.34 mg/L, dibenz[a,h]anthracene of 202 ± 2.86 mg/L, indeno[1,2,3-cd]pyrene of 98.61 ± 1.39 mg/L, and pyrene of 100.6 ± 1.33 mg/L. The simulated organic system of B(a)P was composed of 100 μL PHAs (100 μg/mL) and 900 μL sterile water.

The separation of sixteen kinds of PAHs within 35 min showed good linearity in the range of 0.10–5.00 μg/mL, and the detection limit of B(a)P was 0.90 μg/mL [18, 19].

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.