To prepare cell culture media, 1% penicillin–streptomycin was added to RPMI 1640 and DMEM supplemented with 10% FBS and 5% FBS, respectively. RAW264.7 and HEK293T cells were cultured using RPMI1640 and DMEM media in a humidified incubator maintained at 5% CO2 and 37 °C. Trypsin was used for the subculture of HEK293T cells, and a cell scraper was used to detach RAW264.7 cells from the plate.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.