To evaluate DNA preservation and potential for obtaining genome-wide data, an initial ‘screening’ sequencing run was performed with all five Gjerrild libraries pooled together. The criteria for selecting candidates for deeper sequencing was inspired by previous guidelines [1], excluding samples with an endogenous human DNA content <0.5% and <10% C-T damage in the 5’ends (see below). Furthermore, library clonality and overall sequencing efficiency was evaluated. Libraries from the three best samples (Gjerrild 1,5 and 8) were selected for deeper sequencing with one library sequenced per lane, and the data were merged to sample level as outlined above.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.