The transgenic roots for XTH43-OE, XTH43-RNAi and their appropriate OE control and RNAi controls have been analyzed for differences in cell wall composition using uninfected roots for all analyses. The roots have been crushed in liquid nitrogen in an autoclaved and cooled mortar and pestle. After crushing, the samples have been kept at -80°C and freeze-dried (Labconco, Freezone® 4.5 Plus). The sample weights have been recorded and the samples kept at 4°C until processed for further analyses.

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