Prior experiments have shown XTH43 overexpression leads to a 70–89% decrease (p < 0.05) in H. glycines parasitism as measured by the community-accepted standard representation of the obtained data known as the female index (FI) [48,60]. The FI = (Nx/Ns) X 100, where Nx is the average number of females on the test cultivar and Ns is the average number of females on the standard susceptible cultivar [60]. In the experiments presented here, Nx is the pRAP17-XTH43 (RNAi) transformed root (XTH43-RNAi) and Ns is the pRAP17-ccdB control root (RNAi control) which has been calculated according to our prior published methods [50]. The FI has been calculated as cysts per mass of the whole root (wr) and also cysts per gram (pg) of root according to our published methods [50]. The wr analysis is how the data has been presented historically, not taking into consideration root performance [60]. The pg analysis accounts for possible altered root growth caused by the influence of the RNAi of XTH43. Three biological replicates have been made for the XTH43-RNAi and RNAi control with 10–20 individual transgenic plants serving as experimental replicates, each. A statistical analysis has been performed using the Mann–Whitney–Wilcoxon (MWW) Rank-Sum Test, p ≤ 0.05 cutoff [61,62]. The MWW Rank-Sum Test, as stated, is a nonparametric test of the null hypothesis not requiring the assumption of normal distributions [61].

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