Adult insects of P. stali were collected at a forest edge in the National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan, from which an inbred laboratory strain was established. A mass-reared colony of the strain was used as the source of experimental insects. The insects were reared in plastic containers (150 mm in diameter, 60 mm high) with raw peanuts, dry soybeans, and drinking water supplemented with 0.05% ascorbic acid at 25°C ± 1°C under a long-day regime of 16 h light and 8 h dark as previously described [29].

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