The following high grade chemicals were obtained commercially or as a generous gift: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) (Sigma-Aldrich, St Louis, MO, USA); raloxifene (Cipla Ltd., Goa, India); phosphorous, calcium, alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (TRAP), hydroxyproline (HP), total cholesterol (TC) and triglyceride (TG) kits (Span Diagnostic Pvt. Ltd.); dimethyl sulfoxide (DMSO) and phosphate buffer saline (PBS) (Mediatech Inc., Manassas, VA, USA); xylazine (Indian Immunologicals Ltd., Hyderabad, India); ketamine (Neon Laboratories Limited, Thane, India); diclofenac (Troikaa Pharmaceuticals Ltd., Ahmedabad, India); gentamicin (Abbott, Pitampur, India); DPPH (1,1-diphenyl-2-picrylhydrazyl) (HIMEDIA Co. Ltd., India); ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay kit (Sigma-Aldrich, MO, USA, Catalog Number MAK187); OxiSelect™ TAC Assay Kit (Cell Biolabs, Inc., San Diego, CA, USA; Catalog Number: STA- 360); fetal bovine serum (Mediatech, Manassas, VA); penicillin streptomycin solution 100X (10,000 IU/mL penicillin and 10,000 μg/mL streptomycin) (Mediatech, Manassas, VA) were procured.

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