Organoids were fixed using 2.5% glutaraldehyde in 0.1 M phosphate buffer, osmicated in 1% osmium tetroxide, and dehydrated in ethanol. During dehydration, 1% uranyl acetate was added to the 70% ethanol to enhance ultrastructural membrane contrast. After dehydration, the organoids were embedded in Durcupan, and 70-nm sections were cut on a Leica ultramicrotome, collected on Formvar-coated single-slot grids, and imaged on a Tecnai 12 Biotwin electron microscope (FEI).

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