For each ASD and TDs, about 50 mg feces were mixed with 1000 μL of extract solvent, and samples were transferred to UHPLC-QE Orbitrap/MS analysis. The differential analysis was performed using the ropls package(v1.12.0), and metabolites were with VIP > 1, P-value (T-test) < 0.05 and FC>1.5 were defined as significant different in abundance. The detailed methods have been presented in our previous study.

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