The unigenes were functionally annotated by mapping to different functional protein databases with BLAST software. Predicted unigenes were assigned to the Kyoto encyclopedia of genes and genomes (KEGG), the evolutionary genealogy of genes: non-supervised orthologous groups (eggNOG), and carbohydrate-active enzymes database (CAZy) database using DIAMOND (v0.9.9). The abundances of each functional annotation were the sum of the abundance of annotation of each functional level.

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