About 2 μg DNA per sample was prepared for library construction and then sequenced on the Illumina HiseqX platform. Low quality or contaminated human reads were removed, and the high-quality reads were assembled into contigs using SOAPdenovo (v2.04).79 MetaGeneMark (v2.10)80 was used to predict genes from the assembled contigs. CD-HIT v4.5.881 was used to generate a non-redundant gene catalog. Abundances of the genes were computed by aligning high-quality reads to the reference gene database. For taxonomic identity and functional assignment of unigenes, reads were aligned to the NCBI NR database (e-value≤1e-5) using DIAMOND (v0.9.9).82 The LCA algorithm83 was used to conduct annotation. The detailed methods and results have been presented by our other study.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.