HEK293T cells were fixed, lysed, and sonicated. Precleared lysates were incubated overnight at 4°C with mouse anti-Flag antibody, or control mouse IgG. Immunocomplex was collected, purified, and analyzed by Real-time PCR. Primers used for ChIP-PCR were as followed:

IFNB1: Forward: 5ʹ-gttcttgaattctcaggtcg-3ʹ; Reverse: 5ʹ-cccactttcacttctccctt-3ʹ;

IFNL1: Forward: 5ʹ-ccttcttgatgcagctccca-3ʹ; Reverse: 5ʹ-cacgcaaaatgggcaatcca-3ʹ.

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