Cells were seeded in the 6-well plates. After 12 hours, the cell monolayer was scratched with a sterile 10 μL pipette tip to generate a line-shaped wound. Then the cells were cultured in DMEM without FBS. Forty-eight hours later, images of the scratches were acquired with a digital camera. The scratch areas were quantified using Image‐pro Plus 6.0 software. The experiments were performed in triplicate and repeated 3 times.

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