The statistical analyses were conducted using R software. Randomforest test was performed by the “randomForest” package. Further, we selected the differential SNVs shared among three discovered cohorts based on randomforest results using the Wilcoxon rank-sum test (p < .05), which were considered to be potential biomarkers. Boxplot was shown by the “ggplot2” package. Receiver operator characteristic (ROC) analysis was used to assess the performance of the microbial biomarkers using the “pROC” package in R. The heatmap was constructed using TBtools software.31

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