In order to test for baseline activity, locomotor behavior was measured in 17 × 17-inch square acrylic open-field chambers. Before testing, uniformity of light across the arena was confirmed using a light intensity meter, and the chambers were cleaned with 1% Micro-90 before and between trials. Background white noise (∼72 dB) was used during trials. Mice were placed into the center of the chamber to begin testing, and activity was recorded for 30 min. Data were analyzed in 10-min blocks.

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