The SW480 cells (the interaction time of drugs and cells was 24 h) were lysed with the ice-cold RIPA buffer containing 1% protease inhibitor cocktail for 15 min and then centrifuged. Total protein concentrations of the lysates were assessed using BCA protein quantitation kit. Protein samples were separated by SDS-polyacrylamide gel electrophoresis in 6–12% gels and then transferred onto methanol activated PVDF membranes at 220 mA. The PVDF membranes were blocked with 5% skim milk for 20 min, probed with the indicated primary antibody and blotted with the secondary antibody for 1 h at room temperature. Object proteins were detected by Bio-Rad ChemiDocTM (Hercules, CA) (Lv et al. 2018).

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