To analyze ProA purified samples, 1–2 μg of proteins were loaded onto Novex 4–20% Tris-Glycine gels (Invitrogen, catalog # WXP42026BOXA) in the absence of reducing agent. A PageRuler prestained protein ladder 10‒250kD (Thermo Fisher, catalog # 26620) was included on each gel. After running at 150 V for 1 hour, the gel was stained using InstantBlue Coomassie Protein Stain (Expedeon, catalog # ISB01L), briefly washed with Milli-Q water, and then imaged with a Gel Doc System (Bio-Rad).

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