SW480 cells were incubated with BBR for 24 h, and then trypsinized, washed with PBS, and fixed with 70% of ethanol at 4 °C overnight. After washed twice with PBS, the cells were incubated with 100 μL of RNase A for 30 min and then stained with 1 mL of PI for 10 min in the dark. The cell-cycle distribution was analysed by flow cytometry (Tian et al. 2016).

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