The SW480 cells were treated with different concentrations of BBR for 24 h, and then incubated with 5 µg/mL of rhodamine 123 for 30 min in the dark. After centrifuged at 1500 rpm for 5 min, and washed with cold PBS for three times, the cells were re-suspend in 1000 µL of PBS and analysed by using flow cytometry at 488 and 530 nm, respectively (Tian et al. 2016).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.