The SW480 cells were treated with different concentrations of BBR for 24 h, and then incubated with 5 µg/mL of rhodamine 123 for 30 min in the dark. After centrifuged at 1500 rpm for 5 min, and washed with cold PBS for three times, the cells were re-suspend in 1000 µL of PBS and analysed by using flow cytometry at 488 and 530 nm, respectively (Tian et al. 2016).

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