The cytotoxicity of BBR against cancer cells was assessed by using Cell Counting Kit-8. The assay was performed in triplicate. All cells were seeded in 96-well plate at a density of 5 × 103 cells/well at 37 °C. After cell attachment overnight, each well was treated with different concentrations of BBR or 0.1% DMSO for 24 h, respectively. Then, 10 μL of WST-8 solution was added to each well and the cultures were incubated for another 2 h. The absorbance was read at 450 nm by a SYNERGY microplate reader (Bio Tek, Winooski, VT) (Tian et al. 2016).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.