The human colon cancer cells SW480, the human hepatoma cancer cells HuH7, QGY7701 and QGY7703, the human breast cancer cells MCF-7, the human lung cancer cells A549 and the human acute monocytic leukaemia cells THP-1 were obtained from the Cell Resource Centre, Institute of Life Sciences, Chinese Academy of Medical Science. Berberine (>98% pure) was purchased from Sigma-Aldrich (St. Louis, MO). Tanespimycin (17-AAG) and Vorinostat (SAHA) were from Medchem Express (Monmouth Junction, NJ). L-15 medium, DMEM medium and penicillin/streptomycin were from Gibco (Carlsbad, CA). Foetal bovine serum was from Biological Industries (Cromwell, CT). Hoechst 33342 dye was from Sigma-Aldrich Corp. (St. Louis, MO). Annexin V-FITC was from BD Biosciences (San Jose, CA). Cell Cycle and Apoptosis Analysis Kit was from Beyotime (Nantong, China). Protease inhibitor cocktail and BCA protein quantitation kit were from Thermo Fisher (Rockford, IL).

Antibodies specific for β-actin (#4970), Hsp70 (#4872), IKKβ (#2678), EGFR (#4267), phospho-PI3K (#4228), PI3K (#4257), phosphohospho-Akt (#4060), Akt (#9272), phosphohospho-mTOR (Ser2448) (#5536), phosphohospho-mTOR (Ser2481) (#2974), mTOR (#2983), CDK4 (#12790), cyclinD1 (#2978), LC3B (#3868), Bcl-2 (#2872), BAX (#5023), PARP (#9542), cleaved caspase-3 (#9661), cleaved caspase-9 (#9509), Jagged1 (#70109), Notch1 (#3608), NICD (#4147), Hes1 (#11988) and PTEN (#9188) were obtained from Cell Signaling Technology (Danvers, MA). Anti-Hsp90 (#ab13492) antibodies were from Abcam (Cambridge, UK). Antibodies specific for HDAC1 (sc-7872), HDAC2 (sc-7899), HDAC3 (sc-11417), HDAC4 (sc-11418), HDAC6 (sc-11420) and HDAC8 (sc-11405) were purchased from Santa Cruz Biotechnology (Dallas, TX).

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