PrimeScript™ RT Master Mix (#RR036, Takara, Tokyo, Japan) was used for RNA reverse transcription according to the manufacturer’s instructions if not particularly indicated. Quantitative polymerase chain reaction (qPCR) was performed using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (#RR820, Takara, Tokyo, Japan). The primer sequences for genes analyzed are summarized in Additional file 6: Table S5. The relative expression levels were calculated according to 2−ΔΔCT.

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