Lentiviral vectors containing shRNA SNAP23 (sh-SNAP23) and overexpressing SNAP23 were obtained from Shanghai Qihe Company, with shRNA non-targeted control (sh-NT) and empty vector (Vector) as controls. The designed target sequences were showed in Additional file 1: Table S1. The transduced CRC cells were selected with 5 μg/ml puromycin (Sigma, USA).

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