The samples were prepared in a similar manner with the previous protocol up to the addition of the solvent, where instead of the ethanol:hexane blend, 22.7 mL of pure ethanol were added resulting in a ~ 96% ethanol extraction system at a ratio of 81 mL of solvent per 1 g dry biomass. Extraction took place in the dark, on an agitator for 24 h. The extraction solvent was separated by the spent biomass by a 10 min centrifugation at 10,000 × g. From the supernatant, 11.4 mL were removed into a separate tube, where 11.4 mL of hexane and 5.7 mL 10% NaCl deionized water were added. From the two phases formed, 9 mL of the upper phase were removed into pre-weighted glass tubes and evaporated under N2. The lipid yields were then calculated gravimetrically. Incubated samples were treated in an identical manner.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.