The samples were prepared in a similar manner with the previous protocol up to the addition of the solvent, where instead of the ethanol:hexane blend, 22.7 mL of pure ethanol were added resulting in a ~ 96% ethanol extraction system at a ratio of 81 mL of solvent per 1 g dry biomass. Extraction took place in the dark, on an agitator for 24 h. The extraction solvent was separated by the spent biomass by a 10 min centrifugation at 10,000 × g. From the supernatant, 11.4 mL were removed into a separate tube, where 11.4 mL of hexane and 5.7 mL 10% NaCl deionized water were added. From the two phases formed, 9 mL of the upper phase were removed into pre-weighted glass tubes and evaporated under N2. The lipid yields were then calculated gravimetrically. Incubated samples were treated in an identical manner.

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