Approximately 200 mg of freeze-dried biomass were measured in a precision balance in alumina crucibles and placed in a high temperature furnace (Hochtemperaturofen Supertherm HT04/17, Nabertherm, Germany) for overnight ashing. After removal from the furnace, the samples were let to cool down to room temperature, whereupon they were measured again in the precision balance. The ash content was determined gravimetrically and in duplicate.

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