Verteporfin (#SML0534-5MG), 2′,7′-dichlorofluorescin diacetate (DCFDA, #D6883), monodansylcadaverine (MDC, # D4008), Chloroquine (CQ, #C6528), and propidium iodide (PI; #P4864) were purchased from Sigma;. N-acetyl- L-cysteine (NAC, #47,866) and 3-[4, 5-dimethylthiazol-2-yl]-2,5-di-phenyltetrazolium bromide (MTT, #33,611) were obtained from SRL. LysoTracker Red DND-99 (LTR, # L7528), Enhanced Chemiluminescence (ECL, #32,106) and Antifade mountant (4ʹ-6-diamidino-2-phenylindole, #P36962) were procured from Thermo Fisher Scientific. Lipofectamine 3000 was from Invitrogen (#L3000-001). Primary antibodies were obtained from Santa Cruz Biotechnology (anti-β-actin #sc-69879, anti- GAPDH #sc-365062, anti-p53 #DO-1 sc-126 and anti-HSC70 #B-6 sc-7298); or Abcam (anti-LAMP-2A #Ab18528); or Bio Bharti Life Sciences (anti-p62 #BB-AB0130, and anti-HSP70 #BB-AB0210); or Cell Signaling Technology (anti-LC3B-II #D-11 3868S, anti-ATG-5 #D5F5U 12,994, anti-Rab-7 #D95F2 9367, anti-LAMP-1 #D2D11 9091S, anti-TFEB #D2O7D 37785S, anti-Beclin-1 #D40C5 3495 and anti-Caspase-3 #D3R6Y 14,220); and secondary antibodies- (anti-mouse #7076S and anti-rabbit #7074P2) were from Cell Signaling Technology. Plasmid GFP-RFP-LC3 was kindly provided by Dr. Sovan Sarkar (Birmingham Fellow, University of Birmingham). Ub GFP (#11,928) and mRFPUb (#11,935) plasmids were procured from Addgene. pCMV-Neo-Bam-p53-WT (Addgene Plasmid #16,434), pCMV-Neo-Bam-p53-R273H (Addgene Plasmid #16,439), and pCMV-Neo-Bam Empty Vector (Addgene Plasmid # 16,440) were a gift from Bert Vogelstein.

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