To determine total protein content, a chemical extraction was performed using sodium hydroxide. From concentrated suspension, a volume that contained 5 mg of microalgae biomass was resuspended in 2 mL sodium hydroxide (1 M), followed by 1 h of incubation at 95 °C. After this step and upon reaching room temperature, the sample was centrifuged at 10,000×g for 10 min and the supernatant was processed for protein determination with a modified Lowry method (DC™ Protein Assay, BioRAd) using bovine serum albumin as standard [50].

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