To determine total protein content, a chemical extraction was performed using sodium hydroxide. From concentrated suspension, a volume that contained 5 mg of microalgae biomass was resuspended in 2 mL sodium hydroxide (1 M), followed by 1 h of incubation at 95 °C. After this step and upon reaching room temperature, the sample was centrifuged at 10,000×g for 10 min and the supernatant was processed for protein determination with a modified Lowry method (DC™ Protein Assay, BioRAd) using bovine serum albumin as standard [50].

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.