The rats were euthanized after completing the experiment, anesthetized with an intraperitoneal injection of pentobarbital sodium (150 mg/kg), approved methods for euthanasia by the Animal Care and Welfare Committee in the Zhejiang Chinese Medical University. The small intestine and mesenteric artery were excised and fixed in 10% formalin. After fixing, tissues were embedded in paraffin and cut into 4 μm thick sections. Which were stained with hematoxylin and eosin (Nanjing Jiangcheng Bioengineering Institute, Nanjing, China). Stained sections were visualized under the microscope (100X and 400X).

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