Male Sprague-Dawley (SD) rats, with body weights ranging from 160 to 180 g, were provided by Experimental Animal Center of Zhejiang Province (Certificate no. SCXK 2014–0001, Hangzhou, China). They were acclimatized for 2 weeks before experiments. During this period, all of the rats were fed with standard diet and water. Animals were housed in a room with constant temperature, humidity, and light/dark cycle. All animal care and experimental procedures were approved by the Institutional Animal Care and Use Committee (IACUC) in the Zhejiang Chinese Medical University (permission number: ZSLL-2015-84).

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